The quality of life is mainly measured by multiple conceptual frameworks which neglect the cultural-religious context of the societies. Hence, there is not a unique instrument to assess life quality. The present study strives to develop and validate a quality of life questionnaire in light of Javadi Amoli’s view. In this study, the researcher-made questionnaire has been answered by 400 bachelor students. Javadi Amoli’s books have been consulted for developing the questionnaire. The validity of the questionnaire has been confirmed by content validity, face validity and, construct validity (using factor analysis and measuring correlation of identified components with total questionnaire). After ensuring content validity and consulting Bartlett's test and Kaiser-Mayer-Olkin index, factor analysis has been performed using principal component analysis and varimax rotation. The results oriented toward four dimensions as God, others, self, and nature which explained 36.5% of the variance. The reliability of the questionnaire has been measured using split-half method and Cronbach's alpha, and reported as 0.63 and 0.89, respectively. The reliability of the extracted dimensions has been 0.87, 0.88, 0.80, and 0.81, respectively. Accordingly, findings of the present research acknowledges the questionnaire to be purposeful and efficient.