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Determination of enrofloxacin residue in chicken eggs using FPT and ELISA methods
Razzagh Mahmoudi, Reza Norian,
Volume 5, Issue 2 (6-2015)
Abstract

Serological Investigation of Human Cystic Echinococcosis and Associated Risk Factors in the At-risk Population of Northeast Iran in 2018-2019
Mitra Salehi, Akbar Solati, Ahmadreza Atarodi, Jafar Hajavi, Hossein Nezami,
Volume 14, Issue 2 (3-2024)
Abstract
Background: Hydatidosis is a leading zoonotic parasitic disease causing economic losses and public health concerns worldwide. The current study aims to determine the seroepidemiological status of hydatid cysts (HCs) and assess the relationship between risk factors and this disease in high-risk individuals in Gonabd City, Iran.
Methods: This cross-sectional study was conducted in Gonabad health centers in 2018-2019. Sampling was performed using the census method. A total of 393 serum samples were collected from 259 men and 134 women at risk for HC. A questionnaire was prepared for all participants. Demographic information, such as gender, age, place of residence, education level, participation of children in animal husbandry, the way of washing vegetables, and work experience in animal husbandry were recorded. The levels of immunoglobulin G (IgG) antibodies were measured by enzyme-linked immunosorbent assay (ELISA).
Results: The infection rate was 9.7% for men and 10% for women, showing no significant correlation between gender and hydatidosis. Among the affected population, the prevalence rate was 10.4% in undergraduates and 16.3% in the age group of 20-30. Additionally, individuals with less than ten years of experience in animal husbandry had a prevalence rate of 11.7%. Those who had contact with dogs had a prevalence rate of 7.9%, while individuals who did not use disinfectants to wash vegetables had a higher rate of 12%.
Conclusion: This study highlights the significant prevalence of HCs in at-risk individuals. This indicates the need for effective preventive measures to reduce the burden of infection. The recommendations include washing vegetables with disinfectants to eliminate any potential sources of contamination. Additionally, obtaining identification certificates for livestock dogs and collecting stray dogs can help control the spread of the disease. It is essential to implement a comprehensive public health strategy to address this issue effectively.

Aflatoxin M1 Contamination in Milk From North Khorasan Province: Raw vs Pasteurized vs Sterilized
Mitra Salehi, Akbar Solati, Pezhman Bahari, Mahyar Sharifan, Touhid Valizadeh, Hamidreza Shoraka,
Volume 14, Issue 4 (6-2024)
Abstract
Background: The presence of aflatoxin M1, a toxic, mutagenic, and carcinogenic substance, poses significant health risks. Hence, this study assesses the levels of aflatoxin M1 in raw, pasteurized, and sterilized milk obtained from the North Khorasan Province, Iran, and evaluates its potential impact on consumer health.
Methods: This descriptive-analytical study randomly collected 189 raw milk samples. Additionally, 70 pasteurized and sterilized milk samples from supermarkets in the North Khorasan Province, Iran, were included. All samples were assessed for aflatoxin M1 contamination using the enzyme-linked immunosorbent assay method. Meanwhile, statistical analysis (analysis of variance) was performed on the results.
Results: The Mean±SD concentration of aflatoxin M1 in raw milk from the North Khorasan Province, Iran, was 18.44±34.6 ng/L, while pasteurized and sterilized milk had a higher mean concentration of 42.8±21.54 ng/L. The lowest concentration was found in Bojnord City, Iran, at 9.30±8.91 ng/L, while the highest concentration was detected in Jajarm at 30.70±80.50 ng/L. The mean contamination of aflatoxin M1 was not statistically significant (P=0.42) in most cities of the province. Meanwhile, 6.34% of raw milk and 11.4% of pasteurized milk samples had higher levels of aflatoxin M1 than the maximum limit recommended by the Iran National Standard Organization (INSO) (100 ng/L). However, none of the milk samples exceeded the permissible limit set by the Veterinary Organization of the country (500 ng/L).
Conclusion: The mean concentration of aflatoxin M1 in raw and pasteurized milk from the North Khorasan Province is lower than the European Union (EU) standard of 50 ng/L and the approved limit set by the country’s Veterinary Organization (500 ng/L). Therefore, it is not considered a significant threat to the health of adults.

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