Introduction: Spirituality in one of the most important influencing factor on humans’ health. Specifically in Islam, there is special emphasize about effect of spirituality on humans’ physical and psychological health. One of the very new construct in spirituality is spiritual capital. Current research was carried out with the aim of construction spiritual capital scale and concurrent validation of it with spiritual wel-lbeing in an Iranian population. Materials and Methods: Statistical population was the employees of two work complex in Esfahan city, among them, 277 people were selected by stratified random sampling.‌ Research instruments was researcher made spiritual capital scale and spiritual well being questionnaire (Gomez & Fisher, 2012). Data were analyzed by use of exploratory factor analysis, and computation of reliability coefficient and Pearson correlation coefficient. Findings: Results revealed that spiritual capital scale with twenty four items have five factor structure, named hope and reliance to God (10 items), spiritual valuation (5 items), spiritual influence (4 items), spiritual theology (2 items) and pro-God activity (3 items). Cronbachs’ alpha for five components, which is hope and reliance to God, spiritual valuation, spiritual influence, spiritual theology and pro-God activity was 0.91, 0.85, 0.76, 0.74 and 0.75 respectively. Conclusion: results revealed that there is positive significant relationship between spiritual capital components with personal spiritual well being, environmental spiritual well being and transcendental spiritual well being.